Oral Mucosal Epithelial Cells

Culture & characterisation of limbal epithelial cells & oral mucosal cells.

BACKGROUND & OBJECTIVES Sources of autologous tissue that can functionally replace the corneal epithelium have been considered as an alternative to allogenous limbal transplants for limbal stem cells deficiency (LSCD). The aim of the present study was to compare the characterization of oral mucosa with limbal epithelial cells by markers using reverse transcriptase polymerase chain reaction (RT-...

cultivated limbal and oral mucosal epithelial transplantation

stem cells located at the limbus ,  are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states.  when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...

Molecular characterization of explant cultured human oral mucosal epithelial cells.

PURPOSE To culture and characterize oral mucosal epithelial cells (OMEC) grown on de-epithelialized human amniotic membrane (HAM) to explore their suitability as autografts in patients with bilateral ocular surface disease (OSD) and limbal stem cell deficiency. METHODS Oral biopsy samples were obtained from 20 patients undergoing oral reconstructive surgery, with informed consent and Institut...

Persistence of transplanted oral mucosal epithelial cells in human cornea.

PURPOSE To determine the expression of differentiation and progenitor cell markers in corneal tissues that previously underwent autologous cultivated oral mucosal epithelial transplantation (COMET). METHODS Four eyes from three alkaline-injured patients and one thermally injured patient underwent COMET to promote re-epithelialization or corneal reconstruction. Between 10 and 22 months (mean, ...

A Mucosal Intranet: Intestinal Epithelial Cells